ABSTRACT
Purpose: The purpose of this study is to evaluate the effect of transfer of donor corneal tissue from McCarey朘aufmann (MK) medium to Optisol-GS on corneal endothelium. Methods: This was a prospective, randomized comparative study. Twenty paired human donor corneal tissues of optical quality were retrieved. One tissue of the pair was preserved in Optisol-GS preservative medium (Group A) and other tissue of the pair in MK medium (Group B) at the time of corneoscleral disc excision. Within 12 h of retrieval, each cornea was evaluated using slit-lamp biomicroscopic examination and specular microscopic analysis. Group B corneas were transferred to Optisol-GS medium within 48� h of retrieval. Specular analysis of the paired corneas was repeated 3 h after transferring to Optisol-GS. On day 7 of storage, specular analysis of both the tissues was repeated. Results: The average age of the donor at the time of death was 29 years (16� years). The reduction in endothelial cell count, from baseline, in Groups A and B was 5.5% and 5.8% (P = 0.938) on the 3rd day and 8.2% and 12.6% (P = 0.025) on the 7th day, respectively, postretrieval. The coefficient of variation (CV) increased by 36% (P = 0.021) and hexagonality reduced by 19% (P = 0.007) on day 7. All tissues retained an endothelial cell density higher than the accepted critical level for penetrating keratoplasty. Conclusion: Significant endothelial cell loss was noted while transferring tissues from one medium to another, necessitating the need for reevaluation of transferred tissues before utilization.
ABSTRACT
Hybridization of dentine is identified as a basic bonding mechanism between resin and dentine, hence studying the micro morphology of hybrid layer may explain lower bond strength that has been observed in primary teeth. The aim of the present study was to compare the thickness and micromorphology of Hybrid layer formed in primary and permanent teeth for 3 different durations of dentine etching time (5, 10 and 15 sec) with 10% phosphoric acid as etchant. Thickness and micromorphology of hybrid layer was observed on SEM photographs obtained at 750X. Results indicated that primary teeth presented with a significantly thicker hybrid layer compared to permanent teeth at 10 sec (P<0.05) and 15 sec (P<0.01) of dentine etching. It was concluded that owing to the high reactivity of primary teeth to etchants, the etchants be applied for shorter period on primary dentine compared to permanent dentine for better performance of composite restoration in primary dentition.